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Michael Gonzales
Michael Gonzales

Plant Biotechnology Ebook Free Download



Postgraduate and graduate researchers in molecular biology, genetics, molecular breeding, systems biology, agriculture, crops for human foods, biological sciences, plant science, biotechnology, plant physiology and genetics, plant functional genomics, sustainable growth and economy, agrochemical and biological applications for crop protection; Private companies, spin-offs etc. in plant production; Policymakers, stakeholders, associations of plant growers (e.g. European Plant Science Organization)




plant biotechnology ebook free download



This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics covers functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops.


Industrial Biotechnology summarizes different aspects of plant biotechnology such as using plants as sustainable resources, phytomedical applications, phytoremedation and genetic engineering of plant systems. These topics are discussed from an academic as well industrial perspective and thus highlight recent developments but also practical aspects of modern biotechnology.


  • Presents the recent developments in the utilization of plant resources and systems in modern biotechnology.

  • Highlights academic advances and industrial applications, e.g. plant biomass, plant secondary metabolites and plant tissue culture systems.



This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics covers functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops.


Plant biotechnology by Adrian Slater, Nigel Scott, Mark Fowler, PDF, was published in 2003 and uploaded for 300-level Agriculture and Veterinary Medicine students of National Open University of Nigeria (NOUN), offering CRP305 course. This ebook can be downloaded for FREE online on this page. Plant biotechnology ebook can be used to learn Plant biotechnology, plant genomes, plant tissue culture, plant cell culture media, plant growth regulators, callus, cell-suspension cultures, protoplast, root cultures, shoot tip, meristerm culture, embryo culture, microspore culture, clean gene technology, gene manipulation, herbicide resistance, pest resistance, plant disease resistance, plant-pathogen interactions, natural disease resistance pathways, antimicrobial proteins, RNA viruses, abiotic stress, fruit ripening genetic manipulation, engineering plant protein composition, molecular farming, carbohydrate production, pharming, lipid metabolic engineering, medically related proteins, transgenic crops.


Topics : Crop biotechnology, Plant Tissue Culture, Protoplast Isolation, Protoplast Regeneration, Micropropagation, Doubled Haploid Production, Tissue Culture Media, Adventitious Shoot Proliferation, Axillary Shoot Proliferation, Cryopreservation, Micro Techniques, Ploidy Analysis, Microscopy, Electron Microscopy, Flow Cytometry, Cell Sorting, Plant Histological Techniques, plant cells genetic materials, Plant gene delivery, Gene Silencing, Genetic Engineering applications, Hairy Root Cultures, Bioreactors, Biotransformation, Abiotic Elicitation, Biotic Elicitation, Molecular Farming, Industrial Enzymes production, Biodegradable Plastics production, antibodies production


Topics : Agric biotechnology, plant tissues culture, plant genetics engineering, transgenic animal regulation, artificial insemination, embryo splitting, animal cloning, tissue culture techniques, micro propagation, soft wood, molecular farming, nuclear transfer


In addition, plant tissue culture is considered to be the most efficient technology for crop improvement by the production of somaclonal and gametoclonal variants. The micropropagation technology has a vast potential to produce plants of superior quality, isolation of useful variants in well-adapted high yielding genotypes with better disease resistance and stress tolerance capacities [3]. Certain type of callus cultures give rise to clones that have inheritable characteristics different from those of parent plants due to the possibility of occurrence of somaclonal variability [4], which leads to the development of commercially important improved varieties. Commercial production of plants through micropropagation techniques has several advantages over the traditional methods of propagation through seed, cutting, grafting and air-layering etc. It is rapid propagation processes that can lead to the production of plants virus free [5]. Coryodalisyanhusuo, an important medicinal plant was propagated by somatic embryogenesis from tuber-derived callus to produce disease free tubers [6]. Meristem tip culture of banana plants devoid from banana bunchy top virus (BBTV) and brome mosaic virus (BMV) were produced [7]. Higher yields have been obtained by culturing pathogen free germplasmin vitro. Increase in yield up to 150% of virus-free potatoes was obtained in controlled conditions [8]. The main objective of writing this chapter is to describe the tissue culture techniques, various developments, present and future trends and its application in various fields.


Biotechnology has been introduced into agricultural practice at a rate without precedent. Tissue culture allows the production and propagation of genetically homogeneous, disease-free plant material [37]. Cell and tissue in vitro culture is a useful tool for the induction of somaclonal variation [38]. Genetic variability induced by tissue culture could be used as a source of variability to obtain new stable genotypes. Interventions of biotechnological approaches for in vitro regeneration, mass micropropagation techniques and gene transfer studies in tree species have been encouraging. In vitro cultures of mature and/or immature zygotic embryos are applied to recover plants obtained from inter-generic crosses that do not produce fertile seeds [39]. Genetic engineering can make possible a number of improved crop varieties with high yield potential and resistance against pests. Genetic transformation technology relies on the technical aspects of plant tissue culture and molecular biology for:


Genetic transformation is the most recent aspect of plant cell and tissue culture that provides the mean of transfer of genes with desirable trait into host plants and recovery of transgenic plants [63]. The technique has a great potential of genetic improvement of various crop plants by integrating in plant biotechnology and breeding programmes. It has a promising role for the introduction of agronomically important traits such as increased yield, better quality and enhanced resistance to pests and diseases [64].


Recently successful transgenic plants of Jatropha were obtained by direct DNA delivery to mature seed-derived shoot apices via particle bombardment method [68]. This technology has an important impact on the reduction of toxic substances in seeds [69] thus overcoming the obstacle of seed utilization in various industrial sector. Regeneration of disease or viral resistant plants is now achieved by employing genetic transformation technique. Researchers succeeded in developing transgenic plants of potato resistant to potato virus Y (PVY) which is a major threat to potato crop worldwide [70]. In addition, marker free transgenic plants of Petunia hybrida were produced using multi-auto-transformation (MAT) vector system. The plants exhibited high level of resistance to Botrytis cinerea,causal agent of gray mold [71].


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